Study of the Method to Extract the Flavonoids in Orange Peel

Ke Huiming, Wang Xiaoyi, Liu Lei
(College of Environment and Energy Engineering , Beijing University of Technology, Beijing 100022)

Abstract The supercritical CO₂ modified with ethanol was used to extract biologically anticancer active flavonoids in orange peel. Silica gel column with dichloromethane as the eluting solvent was used to remove the impurities. Analysis of the flavones was carried out by means of reversed-phase high performance liquid chromatography on a C₁₈ column with UV detector at the wavelength of 270nm. A mixture of water and methanol (V(MeOH)/V(H₂O)=65/35) was used as the mobile phase at 30℃. The optimum condition for supercritical CO₂ extraction was found to be: 150mL ethanol at a pressure of 35MPa, and 60℃ for 3h. The determined results showed that the recovery and the selectivity of the supercritical CO₂ extraction were better than that of traditional extraction. The extraction rate of biologically anticancer active flavonoids by supercritical CO₂ fluid extraction was about 2 times as that of traditional solvent extraction. Compared with traditional solvent extraction, the method of supercritical CO₂ extraction has the advantages of short extraction time, high extraction efficiency and no poisonous waste etc.
Key words Supercritical CO₂ extraction, Flavonoids, Solvent extraction, High performance liquid chromatography, Orange peel

客慧明 王小逸 刘 雷
(北京工业大学环境与能源工程能学院 北京 100022)

摘要 建立了一套用超临界CO₂萃取法提取柑橘皮中具有抗癌生物活性的黄酮，用硅胶柱作为预处理柱，以二氯甲烷作为洗脱剂，并用高效液相色谱法测定柑橘皮中黄酮的含量的方法。色谱条件为C₁₈柱，紫外检测器，检测波长为270nm，流动相为甲醇的水溶液(V(甲醇)/V(水))=65/35)，温度30℃。超临界CO₂萃取的最佳条件为：温度为60℃，压强为35MPa，改善剂乙醇150mL，萃取时间为3h。该法提取柑橘皮中的黄酮的萃取率和选择性都好于有机溶剂萃取法，黄酮的萃取率是有机溶剂萃取法的2倍。与传统的有机溶剂萃取法相比超临界CO₂萃取法具有省时、萃取效率高且无有毒废弃物等优点。
关键词 超临界CO₂萃取法 黄酮 溶剂萃取 高效液相色谱 柑橘皮

客慧明 男，33岁，硕士，从事环境科学领域研究。
2003-10-17收稿，2004-04-09接受

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