Applications of Oligonucleotide Aptamers in Analytical Chemistry

Tang Jijun, Shao Ningsheng^#, Xie Jianwei^*
(Institute of Pharmacology Toxicology, ^#Institute of Basic Medicine, Academy of Military Medical Science, Beijing 100850)

Abstract Systematic Evolution of Ligand by Exponential enrichment (SELEX) is a new combinational chemistry methodology for in vitro selection of specific aptamers. Aptamers are artificial nucleic acid ligands that are capable of high-affinity binding to target molecules, such as amino acids, drugs, proteins and other small molecules or ions etc. They are isolated from combinational libraries of synthetic nucleic acid by an iterative process of adsorption, recovery and reamplification. The use of aptamers as tools in analytical chemistry is on the rise due to the development of the SELEX technology. These aptamers have been used in biosensors, flow cytometry, capillary electrophoresis, and affinity chromatography. In this review, were discussed the applications of aptamers as biocomponents in biosensors, and the applications of aptamers as chromatographic stationary phases.
Key words SELEX, Aptamer, Biosensor, Capillary electrophoresis, Affinity chromatography

摘要 指数富集配基的系统进化(SELEX)技术是一种新的组合化学技术，应用人工合成的随机寡核苷酸文库，通过筛选、分离、富集获得能与氨基酸、蛋白、药物、有机小分子、无机离子等靶物质特异性结合的寡核苷酸适配子，且具有高亲和力。随着SELEX技术的发展，寡核苷酸适配子作为一种分析工具，在生物传感器、流式细胞检测、毛细管电泳、亲和色谱等分析化学领域的应用越来越广泛。本文介绍了寡核苷酸适配子作为生物识别元件在生物传感器中的应用以及作为色谱固定相的应用。
关键词 指数富集配基的系统进化 适配子 生物传感器 毛细管电泳 亲和色谱

唐吉军 男，24岁，硕士生，现从事寡核苷酸适配子的筛选。* 联系人，E-mail: xiejw@bmi.ac.cn
国家自然科学基金资助项目(20305013)
2003-12-02收稿，2004-06-11接受

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