Abstract In this paper，the general H-point standard addition method (GHPSAM) for directly determination of VB₂ in green plants is proposed by spectroscopy，and this method can reduce the interferences of other substances. This method allows one to calculate the concentration of an analysis in the presence of unknown compounds, and the analytical process was easy without separateness. To illustrate the reliability of the proposed method, the concentration of VB₂ in simulacrum was determined and satisfactory results were obtained. The concentration of VB₂ in spinach was determined, its recovery and quality control was studied. The results were satisfactory, and the content of VB₂ in spinach was 1.29×10^-6±0.067×10^-6 g/g and the recovery was 98.5%. The accuracy of this method was high. It is considered that this method could be applied and popularized.
Key words General H-point standard addition method, Riboflavin, Chlorophyll, Spectrophotometry

通用H点标准加入法直接测定绿色植物中的维生素B₂

李 蕊¹ 白 燕^1* 吴雅琴^1,2 郭书好¹ 胡中伟¹
(¹广州暨南大学化学系 广州 510632 ² 山西师范大学化学系 临汾 041004)

摘 要 应用通用H点标准加入法有效地消除了分光光度法测定维生素B₂(VB₂)时叶绿素的干扰，可直接用于绿色植物中VB₂含量的测定，无需分离，分析过程简单。通过模拟样品的测定、回收率实验以及质量控制，说明该方法的准确度高。建立了直接测定绿色植物中VB₂含量的新方法，菠菜中VB₂的含量为1.29×10^-6±0.07×10^-6g/g，与文献值相符。平均回收率为98.6%，结果令人满意。
关键词 通用H点标准加入法 维生素B₂ 叶绿素 分光光度法

李 蕊 女，25岁，硕士研究生，现从事天然药物分析与研究。*联系人，E-mail:baiyemail@163.net
2003-10-22-收稿，2004-03-16接受

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