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Separation of Enantiomers of Ibuprofen Esters by Reversed-phase High-performance Liquid Chromatography Feng yanlong, Shi Jiehua #, Li bing *, Jiang feng
# Abstract Tri-(4-methylbenzoate) cellulose chiral stationary phase
(MCTB) was prepared. Separation of enantiomers of ibuprofen esters had been investigated
by reversed-phase high-performance liquid chromatography on the chiral stationary phase.
The experimental results showed that the separation of enantiomers of the methyl, ethyl
and n-propyl esters of ibuprofen could be achieved on the tri-(4-methylbenzoate)
cellulose chiral stationary phase (MCTB) using methanol and water (V(methanol)/V(water)=85/15,)
as mobile phase. Their separation factors were 1.17, 1.29, 1.27, respectively. At the same
time, It was found that the size of the ester group obviously affected the separation
efficiency of their enantiomers on the MCTB chiral stationary phase. The separation of the
ethyl ester was most satisfactory.
冯炎龙 施介华# 李 兵* 江 峰#(浙江林学院理学院 临安 311300 #浙江工业大学药学院杭州 310032) 摘要 合成了三-(4-甲基苯甲酸)纤维素酯(MCTB)手性固定相，用反相高效液相色谱法在该手性固定相上对布洛芬酯的对映体进行分离。实验结果表明，在三-(4-甲基苯甲酸)纤维素酯手性固定相上，以V(甲醇)/V(水)=85/15为流动相能较好地分离布洛芬甲酯、乙酯、丙酯对映体，其分离因子为1.17、1.29和1.27；同时还发现在三-(4-甲基苯甲酸)纤维素酯手性固定相上布洛芬酯的酯基团的大小对其对映体的分离有明显的影响，其中以乙酯最佳。关键词 手性固定相 对映体分离 高效液相色谱 布洛芬酯 冯炎龙 男，38岁，讲师，主要从事分析化学的研究和教学。*联系人2003-06-28收稿，2003-12-06接受 |