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Some Progresses of the Directed Enzyme Evolution Jiang Benguo, Fan Shengdi,
Liu Baoquan Abstract The
directed enzyme evolution is one of the
important bioengineering technologies that was developed rapidly, it is also the necessary
way through which the traits of enzymes，such as thermostabilities, pH tolerance,activities in organic
solvents,substrate specificities and enantioselectivities, are improved and to make
enzymes more suitable to industrial uses,it is also the main way for anzyme researches.In
this paper the significance,the strategy and the main technology ways,the basic demand of
the directed enzyme evolution were reviewed, and the main progresses in the directed
enzyme evolution achieved in recent years were also reviewed. 酶的定向进化 蒋本国 范圣第 刘宝全 摘要 酶的定向进化是发展迅速的重要生物工程技术之一，是改进酶的热稳定性、耐酸碱性、耐有机溶剂性、底物特异性、立体选择性等性质，使酶更适于工业应用的重要途径。定向进化也是研究抗体酶的主要途径。本文综述了酶的定向进化的意义、策略与主要技术手段以及对成功的酶定向进化的基本要求，并综述了在最近几年内，酶的定向进化研究所取得的的主要进展。 蒋本国 男，50岁，副教授，现从事生物化学与有机化学的教学与研究工作。 |