High Performance Chitosan Based Vector Mediated Gene Delivery Zheng Lianying, Xiao Yuliang (Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027) Abstract Efficiency of non-viral gene delivery based on chitosan and chitosan derivatives as DNA-condensing carrier is dependent on a series of factors, such as complex size, complex stability, toxicicy, immunogenicity, protection against DNase degradation and intracellular trafficking of the DNA. The advances in the application of chitosan and chitosan derivatives to non-viral gene delivery and the transfection studies on chitosan and chitosan derivatives as transfection agents, are reviewed. Key words Chitosan, Non-viral gene delivery, Transfection 高性能壳聚糖介导基因载体 郑连英 肖玉良 (浙江大学化学工程与生物工程学系 杭州 310027) 摘要 以阳离子聚合物作载体的非病毒性基因载体的作用效率受到许多因素的影响，如粒子的大小、络合物的稳定性、毒性、免疫原性、保护 DNA 免受 DNase(脱氧核糖核酸酶)降解的能力以及细胞内 DNA 的传递。本文着重介绍了壳聚糖及其衍生物在非病毒基因载体方面的应用以及近年来壳聚糖基因转移载体转染的研究进展。 关键词 壳聚糖 非病毒性基因载体 转染 郑连英 女，57 岁，副教授，现从事生物工程方向的科研和教学工作。 E-mail:zhengly@cmsce.zju.edu.cn 国家自然科学重点基金(50233020)、浙江省自然科学基金(200075)资助项目 2003-04-22收稿，2004-02-13接受 (PDF全文下载c04044)