Some Progresses of the Directed Enzyme Evolution Jiang Benguo, Fan Shengdi, Liu Baoquan (Dalian Nationalities University, Dalian 116600) Abstract The directed enzyme evolution is one of the important bioengineering technologies that was developed rapidly, it is also the necessary way through which the traits of enzymes，such as thermostabilities, pH tolerance,activities in organic solvents,substrate specificities and enantioselectivities, are improved and to make enzymes more suitable to industrial uses,it is also the main way for anzyme researches.In this paper the significance,the strategy and the main technology ways,the basic demand of the directed enzyme evolution were reviewed, and the main progresses in the directed enzyme evolution achieved in recent years were also reviewed. Key words Enzyme, Directed evolution, Trait, Improve 酶的定向进化 蒋本国 范圣第 刘宝全 (大连民族学院生物工程系 大连 116600) 摘要 酶的定向进化是发展迅速的重要生物工程技术之一，是改进酶的热稳定性、耐酸碱性、耐有机溶剂性、底物特异性、立体选择性等性质，使酶更适于工业应用的重要途径。定向进化也是研究抗体酶的主要途径。本文综述了酶的定向进化的意义、策略与主要技术手段以及对成功的酶定向进化的基本要求，并综述了在最近几年内，酶的定向进化研究所取得的的主要进展。 关键词 酶 定向进化 性质 改进 蒋本国 男，50 岁，副教授，现从事生物化学与有机化学的教学与研究工作。 2003-03-04收稿，2003-11-04接受 (PDF全文下载c04035)