Electrochemical Behavior of Nitrate Reductase Incorporated on Pyrolytic Graphite in Chitosan Films Chen Xiaoxia1,2, Hu Jinbo1,2, Tu Shujie, Shao Huibo* (1Department of Chemistry, Capital Normal University, Beijing 100037;) (2Department of Chemistry, Beijing Normal University, Beijing 100875) Abstract Nitrate reductase (NR) and chitosan (CS) were immobilized on pyrolytic graphite (PG) electrode, which gave direct electrochemistry response. Cyclic voltammetry (CV) of CS/NR films showed a pair of well-defined and nearly reversible redox peaks at about -0.430 V vs. SCE at pH 7.0 phosphate buffers. The pH of the solution strongly affected the direct electron transfer of CS/NR films. EDTA was proposed as a stimulant for the system. Key words Nitrate reductase, Chitosan, Cyclic voltammetry, Electrochemical behavior 硝酸还原酶在壳聚糖修饰石墨上的电化学行为** 陈晓霞 1,2 胡劲波 1,2 屠淑洁 邵会波* (1首都师范大学化学系 北京 100037； 2北京师范大学化学系 北京 100875) 摘 要 将硝酸还原酶(NR)涂敷在壳聚糖(CS)薄膜修饰热解石墨(PG)电极的表面，得到 CS/NR 薄膜电极，在 pH 为 7.0 的缓冲溶液中的循环伏安(CV)图上，呈现一对可逆性良好的还原氧化峰，其峰电位在-4.30V(vs.SCE)左右。研究表明，该体系中的 NR 活性受溶液酸度影响很大，EDTA 有可能成为良好的电子转移促进剂。 关键词 硝酸还原酶 壳聚糖 循环伏安法 电化学性质 陈晓霞 女，25 岁，硕士生，现从事分析化学研究。*联系人 国家自然科学基金(29973026)和北京市自然科学基金(2992007)资助项目 2003-11-20收稿，2004-03-01接受 (PDF全文下载c04092)