The Determination of Myrica Rubra Flavonoid by Microcolumn High Performance Liquid Chromatography Hu Qiufen 1,2, Yang Yaling 1, Huang Zhangjie 2, Yang Guangyu 2, Yin Jiayuan 2 (1 Department of Chemistry, Yuxi Teacher’s College, Yuxi 653100) (2 Department of Chemistry, Yunnan University, Kunming 650091) Abstract A microcolumn high performance liquid chromatography (HPLC) method for the determination of flavonoid in myrica rubra leaf and bark was studied. The flavonoid was separated on a Waters XterraTM RP18 (1.0´ 30mm, 2.5m m) chromatographic column, with methanol and 0.05mol/L potassium dihydrogen phosphate buffer solution as mobile phase at a flow-rate of 0.2mL/min, and monitored with the photodiode array detector at 360nm. The recoveries of the flavonoid are 97%～103%, and relative standard deviations are 1.6%～2.2%. Key words Microcolumn high performance liquid chromatography, Solid phase extraction, Flavonoid, Myrica rubra 微柱高效液相色谱法测定杨梅黄酮类物质 胡秋芬 1,2 杨亚玲 2 黄章杰 2 杨光宇 2 尹家元 2 (1玉溪师范学院化学系 玉溪 653100 2云南大学化学系 昆明 650091) 摘 要 经固相萃取预分离，由微柱高效液相色谱法测定了几种杨梅树叶和树皮中的黄酮类物质。杨梅中主要的黄酮成分在 2.0min 内可达到基线分离；用紫外二极管矩阵检测器检测。方法标准回收率为 97％～103%，相对标准偏差为 1.6％～2.2%。 关键词 高效液相色谱 固相萃取 杨梅 黄酮 胡秋芬 女，30 岁，副教授，从事分析化学教学研究工作。E-mail: huqiufena@163.com 云南省教育厅(0211143)和玉溪师范学院(020024)科研基金资助项目 2003-11-30收稿，2004-03-15接受 (PDF全文下载c04094)