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Self-assembled Monolayers for Electrochemical Enzyme-sensors Liu Huihong, Li Jun, Chen Xiantang Abstract The simplicity and adaptability of
self-assembled monolayers (SAMs) and the control over biomolecule surface orientation have
begun to receive attention as a method of constructing electrochemical enzyme-sensors.
This review provides the mechanism of forming SAMs, highlights two immobilization methods,
the modification of electrode surfaces and the engineering of the enzymes, which can be
used for constructing enzyme electrodes, and describes the recent development of
fabricating electrochemical enzyme-sensors using self-assembled monolayer technology.
Further research directions are suggested. 自组装膜技术构筑电化学酶传感器 刘慧宏 李俊 陈显堂(襄樊学院化学系 湖北襄樊 441053) 摘要 自组装膜以其制备简便、结构易控、重现性好、具有良好的生物兼容性、能设计和控制生物分子的取向等特点，用于构筑电化学酶传感器，引起了广泛的关注。本文阐明了自组装膜形成的机理，描述了自组装膜技术在电极表面固定酶的两种方法，即修饰电极法和修饰酶法，综述了自组装膜技术应用于构筑电化学酶传感器的最新进展，并展望了其发展趋势。关键词 自组装膜 生物传感器 电化学 酶 刘慧宏 男，38岁，副教授，主要从事生物电化学和生物传感器的研究。湖北省教育厅资助项目(2001B69002) 2002-06-06收稿，2002-10-15修回 |