Application of Chromatography in the Separation and Analysis of Collagen(Ⅱ)

Wang Bia, Jia Dongyinga, Shu Zibina, Zhang Mingranga,b, Wang Shenyua,b
(aThe Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu, 610065)
(bResearch Center for Biomass of Sichuan University, Chengdu, 610065)

Abstract Advance in the application of chromatography in the separation and analysis of collagen and its cyanogens bromide fragments was reviewed. In addition, the application of capillary electrophoresis in the separation and analysis of collagen a-chain and cyanogen bromide fragments was introduced. Capillary electrophoresis is now successfully used to quantitatively analyze collagen marker peptide.
Key words Chromatography, Collagen, Cyanogen bromide peptides, Capillary eletrophoresis

 

色谱法在胶原蛋白分离、分析中的应用(Ⅱ)

王 碧a 贾冬英a 舒子斌a 张铭让a,b 王坤余a,b*
(a四川大学皮革化学与工程教育部重点实验室 b四川大学生物质研究所 成都 610065)

摘要 介绍了国外研究者利用凝胶过滤、离子交换和反相色谱等技术分离、分析胶原溴化氰肽的研究进展以及毛细管电泳法在分离、分析胶原a-链和溴化氰肽等中的应用情况。
关键词 色谱法 毛细管电泳法 胶原蛋白 溴化氰断裂肽


王碧 女,38岁,博士生,副教授,现从事胶原蛋白的色谱分离研究。 *联系人
国家自然科学基金资助项目(298877017)
2002-03-15收稿,2002-08-30修回

(PDF全文下载c03008)