Synthesis of N-Saccinimidyl-chlormercuriisonicotinizute

Zhou Jimeng,  Luo Wen^#
(Institute of Nuclear Science and Technology, Sichuan University, Chengdu 610064)
(^#Department of Biological Engineering, Chengdu University, Chengdu 610081)

Abstract By virtue of isonicotinic acid as precursor compound, N-saccinimidy lisonicotinizute has been synthesized through eletrophilic reaction and then being esterified, one kind of conjugate reagent adapted to all kinds of protein can be obtained. Moreover, a quick and convenient way of labell antibody has been estabished, combining this conjugate reagent with the technique of the fixed phrase separation. The rate of its protein labell thus can reach 40%～60% averagely.
Key words Conjugate reagent, Labell-protein, Synthesis, Redioimmunoassay

偶联剂汞代异烟酸-N-丁二酰亚胺酯的合成研究

周继萌  罗 雯^#
(四川大学原子核科学技术研究所 成都 610064  ^#成都大学生物工程系 成都 610081)

摘要 用亲电取代法通过异烟酸与氧化汞直接反应，合成汞代吡啶羧酸再进行酯化，制得一种适合于各种蛋白质碘标记的偶联剂：氯化汞代异烟酸-N-丁二酰亚胺酯。该试剂结合固相分离技术来建立一简便的标记抗体途径。其蛋白质标记率可达40%～60%。
关键词 偶联剂 标记蛋白质 合成 放射性免疫分析