Methods of Polyethylene Glycol(PEG) Activation and Analysis Methods for Polyethylene glycolated Protein

Qin Haina,  Xiu Zhilong^*
(The Department of Biotechnology of Dalian University of Technology  Dalian  116023)

Abstract Polyethylene Glycol(PEG) modification of protein drugs is a practical method to reduce the immunogenicity and to prolong the half life of protein in plasma. In this article, we introduce the methods of preparation, analysis and separation of modified proteins, and put forward some advices about the appropriate methods for different protein analysis.
Key words  Polyethylene glycolated,  Modification,  Activation,  Separation,  Analysis

聚乙二醇修饰蛋白质的活化与检测方法

秦海娜  修志龙^*
(大连理工大学生物工程系  大连  116012)

摘要  聚乙二醇共价修饰蛋白质药物可以消除蛋白质药物的抗原性及免疫原性，延长小分子多肽在体内的半衰期。本文对该项技术最近的制备、分离、分析方法及各种方法的优缺点作一介绍，并对不同蛋白质需采用的分析方法提出了一点建议。
关键词  聚乙二醇   修饰  活化  分离  分析

秦海娜  女，23岁，硕士生，目前从事聚乙二醇修饰水蛭素的研究工作。*联系人E-mail: zlxiu@mail.dlptt.ln.cn
2003-01-13收稿

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