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Advance in Studies of the Catalytic Performance of Enzyme in Organic Media and Reverse Micelles Wang Dan, Huang Xirong*, Li Yuezhong#, Qu Yinbo# Abstract Enzyme
plays a key role in biosynthesis and biotransformation. Its catalytic properties depend on
the structure of enzymic protein as well as on the microenvironment it experienced.
Nonaqueous Enzymology and Micellar Enzymology are new branches of enzymology in recent
decades, the research aim is to study the effect of organic solvent and reverse micelles
on the catalytic properties of an enzyme. Surfactant, amphile, is viewed as both the mark
to distinguish between them and the bridge to connect them. In addition to make a brief
introduction of these two research fields, this review article puts emphasis on recent
advances in modification of enzyme by surfactant and immobilization of enzyme in
water-in-oil microemulsion. 有机溶剂及反胶束中的酶催化性能 王
丹 黄锡荣*
李越中＃ 曲音波＃ 摘 要 酶是生物合成与转化的关键因素，酶催化性能既取决于酶蛋白分子自身结构，又取决于介质微环境。非水酶学和胶束酶学是近二三十年来兴起的酶学研究新领域，主要研究有机溶剂和反胶束中酶的催化性能并探索其潜在应用。表面活性剂既是区分这两个分支领域的标志，又是联系两者的桥梁。本文在对这两个领域的研究概况作一简要介绍的同时，着重介绍了表面活性剂修饰酶、微乳液凝胶固定化酶等研究进展。 王丹 女，23岁，硕士生，现从事胶束酶学研究工作。*联系人 E-mail:xrhuang@sdu.edu.cn |