Enantiometric Separation of Dipeptides with Hydroxypropyl-b-Cyclodextrin by Micellular Electrokinetic Chromatography

Abstract The enantiomeric separation of dipeptides with one and two stereogenic centers by micellular elctrokinetic chromatography (MEKC) is described. The dipeptides were precolume derivatized with a fluorescence tagging reagent, naphthalene-2,3-dialdehyde (NDA). In the presence of hydroxypropyl-b-cyclodextrin (HP-b-CD) as the chiral selector and methanol as organic modifier, NDA-tagged dipeptid enantiomers were well resolved by MEKC. However, without enantiomeric separation was achieved by using capillary zone electrophoresis or in the absence of methanol.
Key words Micellular electrokinetic chromatography, Chiral separation, Dipeptide, Hydroxypropyl-b-cyclodextrin

赵书林 李舒婷
(广西师范大学化学化工系 桂林 541004)

摘要 以羟丙基-b-环糊精为手性选择剂，甲醇为有机修饰剂，对四种含有一个和两个手性中心的二肽对映体的毛细管胶束电动色谱分离进行研究。结果表明，二肽经萘-2.3-二甲醛柱前衍生后，各种旋光异构体能够由毛细管胶束电动色谱完全分离，而采用毛细管区带电泳或无甲醇存在时，均不能达到手性拆分。
关键词 电动色谱 手性分离 羟丙基-b-环糊精 二肽

赵书林 男，45岁，教授，主要从事毛细管电泳生物分析和有机分析试剂的合成研究。
广西科学基金资助项目(0236013)和广西教育厅科学基金资助项目
2002-09-10收稿，2003-05-19修回

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