Preparation of Water-soluble Chitosan and Study of Scavenging Superoxide Anion
Radical Activity of Chitosan and its Copper Complex

Yin Xueqiong, Lin Qiang, Zhang Qi, Feng Yuhong, Yang Lichun
( Department of Chemical Engineering, Hainan Univertsity, Haikou 570228)

Abstract A new method that was called metal-coordination controlled degradation for chitosan depolymerization was reported here. With this method, water-soluble chitosan with narrow MW distribution could be obtained at room temperature. The scavenging superoxide anion radical (O2-) activity of the obtained chitosan(LMC) and its complex with Cu(Ⅱ) was investigated. The results showed that when mass concentration of samples was 0.5×10-2g/mL, the scavenging O2- activity of LMC and LMC-Cu(Ⅱ) were 80.3%, and 84.1% respectively, but those of high molecular weight chitosan(820kD) and D-Glucosamine were only about 13% and 9.5%. The scavenging activity of LMC and its complex decreased with the decrease of their mass concentration.
Key words Water-soluble chitosan, Preparation, Complex, Superoxide anion radical, Scavenging activity

　

水溶性壳聚糖的制备及其对超氧阴离子清除活性的研究**

尹学琼 林强 张岐 冯玉红 杨丽春
(海南大学理工学院化工系 海口 570228)

摘要 提出了一种新的壳聚糖降解法棗壳聚糖金属配位控制降解法，该方法能在室温，以比直接氧化法更快的速度将壳聚糖降解为分子量分布更窄的水溶性壳聚糖。以比色法对所得水溶性壳聚糖及其Cu(OAc)2·H2O配合物进行了抗超氧阴离子活性研究，结果显示水溶性壳聚糖及其配合物对超氧阴离子均具有明显的清除活性。质量浓度为0.5×10-2 g/mL时，壳聚糖对超氧阴离子的清除率达80.3%，铜配合物的清除率为84.1%；相同条件下，高分子壳聚糖和单糖没有明显的清除作用，清除率仅为13%、9.5%。随着样品质量浓度降低，水溶性壳聚糖及其金属配合物对的清除活性逐渐下降。
关键词 水溶性壳聚糖 制备 壳聚糖金属配合物 超氧阴离子

尹学琼 男 E-mail:xq88@21cn.com
国家自然科学基金资助项目(20061001)及海南省海南省教育厅高等学校科研基金资助项目
2002-12-26收稿，2003-03-29修回

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