Fluorescence and UV-Vis Absorption Spectroscopic Studies on the Interaction between DNA and Schiff-base Complexes Derived from Methyl-substituted Salicylal D-Glucosamine

Huang Yonghua, Shen Haoyu^#, Long Shu
(Department of Core Curriculum, Hunan Light Industry College, Changsha, Hunan, 410015)
(^#Department of Chemistry, Fudan University, Shanghai, 200433)

Abstract The interactions between DNA and three Schiff base ligands derived from methyl-substituted salicylal D-glucasamine (3-MSG, 4-MSG, 5-MSG) and their 21 metal complexes ML(ML, M = Cu(II), Zn(II), Fe(II), Fe(III),Co(II), Co(III), Ni(II); L = 3-MSG, 4-MSG, 5-MSG) have been investigated by fluorescence and UV-Vis absorption spectrophotometry. The fluorescence spectrophotometry studies show that 5 of the 21 complexes combined with DNA strongly. The Scatchard plots of the 5 complex-DNA systems have been further studied by UV-Vis absorption spectrophotometry. The combining constants of the interaction between DNA and the 5 complexes are 16.25´ 10⁴, 7.75´ 10⁴, 12.5´ 10⁴, 8.75´ 10⁴, 8.86´ 10⁴ L·mol^-1 for Cu(3-MSG), Cu(4-MSG), Cu(5-MSG), Co(3-MSG) and Co(5-MSG) respectively.
Key words DNA, Deoxyglucosamine Schiff-base complexes, Fluorescence spectrometry, Uv-vis absorption spectrometry, Scatchard plot

氨基葡萄糖席夫碱金属配合物与DNA的作用

黄永华 沈昊宇^#* 龙姝
(湖南轻工业高等专科学校基础部 长沙 410015 ^#复旦大学化学系 上海 200433)

摘要 报道了3种甲基取代水杨醛D-2-氨基-2-脱氧葡萄糖席夫碱及其21种金属配合物与DNA相互作用的荧光光谱和紫外吸收光谱。通过研究化合物-溴化乙啶-DNA体系的荧光光谱，从中筛选出5种与DNA有较强相互作用的配合物。进一步研究这5种配合物与DNA作用的紫外吸收光谱，得到配合物与DNA作用的Scatchard曲线，推导出这5种配合物与DNA作用结合常数分别为16.25´ 10⁴, 7.75´ 10⁴、12.5´ 10⁴、8.75´ 10⁴、 8.86´ 10⁴ L·mol^-1。
关键词 DNA 氨基葡萄糖席夫碱金属配合物 荧光光谱 紫外吸收光谱 Scatchard曲线

黄永华 男，31岁，讲师，从事生物无机研究。 *联系人
2001-09-30收稿，2001-12-25修回