Study on the Chemical Components of Antheraea Pernyi Crust and Its Morphologic Structures Ni Hong, Liang Hui# (School of Life Science, Hubei University, Wuhan 430062) (#Laboratory of Electron Microscope of Hubei Medicine College, Wuhan University, Wuhan 430071) Abstract The chemical composition of pupa crust of Antheraea pernyi has been determined, and the microstructures of pupa crust have been studied by scanning electron microscope. The results show that the major portion of pupa crust is composed of fat, protein, chitin and inorganic salts. Their corresponding contents are 12.81%, 32.60%,27.30% and 6.86%, respectively. There is regular burry-shape structure on the outer surface of pupa crust. The burries are composed of chitin-protein complex which is filled with granules of inorganic salts. The inner surface of pupa crust shows a craggy hill shape. The proteins in pupa crust connect with silk-like chitin and they are assembled layer by layer. It provides the theory basis for isolating chitin from pupa crust of Antheraea pernyi. Key words Antheraea pernyi crust, Chitin, Morphologic structure, Scanning electron microscope 柞蚕蛹皮的化学组分及其形态结构 倪 红 梁 惠# (湖北大学生命科学学院 武汉 430062 #武汉大学湖北医学院电镜室 武汉 430071) 摘要 采用化学分析方法对柞蚕蛹皮的化学组分进行了分析，并采用扫描电镜对其中的主要组分的形态结构进行了显微表征。研究结果表明，柞蚕蛹皮的主要成分为油脂、蛋白质、几丁质和无机盐。它们分别占蛹皮干重的 12.81%、32.60%、27.30%和 6.86%。柞蚕蛹皮外表面呈规整“毛刺”状结构，“毛刺”主要由几丁质/蛋白质复合物组成，颗粒状无机盐均匀分布在该复合物的表面上。其内表面为光滑的“丘陵”形貌。蛹皮中的蛋白质和蚕丝状几丁质相结合，呈层状分布。这为制定提取柞蚕蛹几丁质的工艺路线提供了理论依据。 关键词 柞蚕蛹皮 几丁质 形态结构 扫描电子显微镜 倪红 女，38 岁，硕士，讲师，主要从事生化物质的分离工艺和开发研究工作。 湖北省科技厅重点科技项目(971P1807) 2002-04-01收稿，2002-05-09修回 (PDF全文下载c02099)