Progress in the Studies of Enzyme Immobilizatin and Applications of Immobilized Enzyme Diao Yinghui, Fu Shiyu, Yu Huisheng (Laboratory of Celluose and Lignocellulosic Chemistry, Guangzhou Institute of Chemistry Chinese Academy of Sciences) Abstract Enzyme immobilization benefits the separation of reactant from enzyme and the reuse of enzyme. Some research developments on enzyme immobilization including the methods, functional groups useful for immobilization and mass transfer effect are reviewed. Some applications of immobilized enzyme on biosensor, organic reaction and environmental protection have also been discussed. Key words Immobilized enzyme, Enzyme Carrier, Mass transfer effect 生物酶的固定化及其应用 刁颖辉 付时雨* 余惠生 (中科院广州化学研究所纤维素化学开放实验室 广州 510650) 摘要 固定化酶有利于实现生物酶与反应物的分离及酶的反复利用，有很好的应用前景。文章从固定化酶的方法类别，用于固定化的功能基团，固定化后传质作用的影响等方面进行综述，并介绍了固定化酶近些年在生物传感器，有机反应，环境保护领域的应用。 关键词 固定化酶，生物酶载体，传质作用 刁颖辉 女，24岁，硕士生, 现从事生物化学与环境化学的研究。 *联系人 Email:fusy@mail.gic.ac.cn 广东省自然科学基金资助项目(2KM064035, 990745) 2001-08-03收稿，2001-11-07修回 (PDF全文下载c02033)