Rapid Determination of Organophosphorus Pesticide Residues in Vegetables using a Plant-hydrolase Inhibition Technique with Microwave Extraction Zhong Shuming, Yuan Dongxing, LI Quanlong, Zhang Jun (Environmental Science Research Center, Research Lab of SEDC of Marine Environment, Xiamen University, Xiamen 361005) Abstract A technique of plant-hydrolysis inhibition with microwave extraction has been developed to determine organophosphorus pesticide residues in vegetables. The chlorophyll existing in vegetables is removed using a mini-column filled with Celite 545. The plant-hydrolysis enzyme is inactivated under microwave irradiation. The extraction efficiency with microwave is higher than those of shaking and heating extraction methodS. Key words Vegetables, Organophosphorus, Microwave extraction, Plant-hydrolase 微波萃取 —植物酶抑制法快速检测蔬菜中有机磷农药残留 钟树明 袁东星* 李权龙 张 军 (厦门大学环科中心 厦门大学海洋环境科学国家教育部重点实验室 厦门 361005) 摘要 应用植物酶抑制法检测蔬菜中的农药残留。用小柱对样品进行预处理，可去除叶绿素和杂质的干扰、提高方法灵敏度。采用微波辅助萃取，能有效地去除蔬菜本身含有的植物酶对显色反应的影响，提高萃取效率和灵敏度。所建立的方法快速、灵敏、可靠。 关键词 蔬菜 有机磷农药 微波萃取 植物酶 钟树明 男，25岁，硕士生，现从事环境分析化学研究工作。 *联系人 国家自然科学基金(20077022)资助项目 2001-06-18收稿，2001-07-27修回 (PDF全文下载c02002)