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| 基于纳米酶酶级联增效CDT/超声/ 免疫协同治疗乳腺癌的实验研究 |
| Experimental study on the synergistic treatment of breast cancer based on nanoenzyme cascade enhancement of CDT/ ultrasound/immunotherapy |
| 投稿时间：2026-04-12 修订日期：2026-05-12 |
| DOI： |
| 中文关键词: 纳米酶 化学动力疗法；超声 免疫治疗 乳腺癌 谷胱甘肽耗竭 |
| 英文关键词:Nanozyme Chemodynamic therapy (CDT) Ultrasound Immunotherapy Breast cancer Glutathione depletion |
| 基金项目:重庆市自然科学基金 （CSTB2024NSCQ-MSX0658） |
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| 中文摘要: |
| 本研究构建了一种多功能纳米酶HSA-GOx-CuO，它可以通过酶级联反应自我供给H?O?，耗竭谷胱甘肽（GSH），从而增强氧化应激，激活抗肿瘤免疫。体外实验结果表明，联合超声处理后，HSA-GOx-CuO可有效消耗谷胱甘肽并提升细胞内活性氧水平（p ＜ 0.05）；同时能够促进树突状细胞成熟，其CD80?CD86?双阳性细胞比例为对照组的2.4倍（p ＜ 0.01）。体内抑瘤实验表明，依托肿瘤组织增强渗透滞留效应，该纳米酶可有效富集于肿瘤部位，肿瘤抑制率可达92.8%，且生物相容性优异。本研究构建的级联纳米平台整合饥饿治疗与GSH耗竭策略，实现了化学动力学治疗、超声治疗及免疫治疗的协同抗肿瘤作用，为乳腺癌精准治疗提供了一种安全可行的新型纳米制剂。 |
| 英文摘要: |
| This study developed a multifunctional nanoenzyme, HSA-GOx-CuO, which can self-supply H?O? and deplete glutathione (GSH) through an enzyme cascade reaction, thereby enhancing oxidative stress and activating anti-tumor immunity. In vitro experiments demonstrated that ultrasound-activated HSA-GOx-CuO effectively depleted glutathione and elevated intracellular reactive oxygen species (ROS) levels (p < 0.05). Notably, it promoted dendritic cell maturation, with the CD80?CD86? double-positive cell population increasing 2.4-fold compared to the control group (p < 0.01). In vivo tumor inhibition studies revealed effective tumor accumulation via the enhanced permeability and retention (EPR) effect, achieving an exceptional tumor suppression rate of 92.8% with excellent biocompatibility.This integrated nano-platform combines starvation therapy with glutathione depletion strategy, achieving synergistic therapeutic outcomes through combined chemodynamic therapy, sonodynamic therapy, and immunotherapy. The developed system presents a safe and effective nanotherapeutic approach for precision breast cancer treatment. |
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